Discover how TriAltus achieved >99% purity of multi-subunit RNA polymerases using a single-step CL7/Im7 system—no polishing steps, no compromise. Perfect for structural biology, CRISPR, and high-throughput assays.
"Unlocking the Magic of CL7/Im7 Protein Purification: A Game-Changer for Scientists" is an article designed to inspire and inform fellow protein scientists about the groundbreaking CL7/Im7 protein purification system.
Choosing the right reagents is crucial when preparing to conduct affinity protein purification. After you’ve chosen a tag system to use, the next step is to choose an appropriate resin. This blog post will outline some of the differences between 4B and 6B agarose resin and how to choose the right resin for your needs.
Eluting your protein of interest is the final step in affinity chromatography before obtaining a pure, useful sample for further study. Common methods include elution using proteolytic elution, low pH elution, and denaturant elution. An alternative to these options is what’s known as Gentle Elution Buffer, or 3.6 M MgCl2 pH 6.6.
Human growth hormone (hGH), also known as somatropin, is a hormone secreted by the pituitary gland. This blog post gives an overview of the uses and features of hGH, as well as its optimized soluble purification using the CL7/Im7 system.
Improvements on the methods and manner of delivery of ribonucleoprotein (RNP) complexes have been made in order to improve efficiency and minimize off-target effects. This blog post reviews the current state of RNP complex delivery systems.
Low protein yield is a common issue in protein purification, especially when it comes to isolating large or complex structures. In lieu of using denaturants to elute protein from an affinity column, protease elution can be a welcome alternative to give tag-free protein.
Biosimilars are functionally significant proteins with therapeutic potential. This blog post highlights the purification of several biosimilar proteins using the CL7/Im7 system.
Membrane proteins are difficult to purify due largely to their hydrophobic regions. When the proteins are in solution, these exposed regions can form strong non-specific interactions with each other, cytoplasmic proteins, or nucleic acids.
Multi-subunit proteins present roadblocks because of their size and the expression of multiple units. In this blog post, we discuss the one-step purification of ttRNAP and mtRNAP, two bacterial RNA polymerases, using the CL7/Im7 system.
