This blog post about pH in the context of protein purification is the third in a series about optimizing conditions for protein purification. Although pH is most commonly seen as an issue in ion-exchange chromatography, it also plays a critical role in affinity tag protein purification.
When conducting affinity purification of a protein, there are many variables to consider in order to optimize purity and yield. One such condition to control is high salt loading, which decreases impurities in the final product.
