Low protein yield is a common issue in protein purification, especially when it comes to isolating large or complex structures. In lieu of using denaturants to elute protein from an affinity column, protease elution can be a welcome alternative to give tag-free protein.
Protein fusion tags make affinity chromatography possible for many proteins, particularly those for which no affinity-quality antibody is available. In addition to improving conditions for purification, tags can facilitate detection and protein interaction studies.
