Discover how TriAltus achieved >99% purity of multi-subunit RNA polymerases using a single-step CL7/Im7 system—no polishing steps, no compromise. Perfect for structural biology, CRISPR, and high-throughput assays.
Multi-subunit proteins present roadblocks because of their size and the expression of multiple units. In this blog post, we discuss the one-step purification of ttRNAP and mtRNAP, two bacterial RNA polymerases, using the CL7/Im7 system.