Simplify protein purification with the new CL7 Affinity Tag and its Im7 Ligand
The new CL7/Im7 Affinity Tag System, exclusively available from TriAltus, is designed to take the hard work and uncertainty out of protein purification. Ideal for scientists with little protein purification experience as well as labs with difficult-to-isolate proteins such as membrane proteins and multi-subunit complexes, TriAltus’ CL7/Im7 Affinity System does everything you wished a His-tag could do and more.
The TriAltus Bioscience CL7/Im7 purification system has helped us combat solubility and purification issues with our protein of interest. We have tried various other systems and constructs to produce our protein of interest, none of which have compared to the results we have seen with this system. It is easy to use, from cloning to purification, and the team at TriAltus is very knowledgeable if any questions come up along the way. Thanks TriAltus!
Research Associate III, Cincinnati Children's Hospital
The cost per item is amazing. I’ve reused the resin multiple times and haven’t seen any loss of binding. Furthermore, using an FPLC column, I have the ability to purify a protein in a couple of hours. The usability is great. Using CL7 we were able to purify a protein that we’ve had trouble with in the past.
PhD Candidate, University of Chicago
CL7/Im7 Affinity Tag System
See why you should be using the CL7/Im7 Affinity Tag System
Choosing the right reagents is crucial when preparing to conduct affinity protein purification. After you’ve chosen a tag system to use, the next step is to choose an appropriate resin. This blog post will outline some of the differences between 4B and 6B agarose resin and how to choose the right resin for your needs.
Different cell expression systems offer certain benefits and drawbacks to yield and post-translational modification. TriAltus has expanded our plasmid offerings to include BacMam vectors so that the CL7/Im7 affinity purification system can be used in a wider scope of applications.
FLAG tag (DYKDDDDK) is an epitope tag widely adopted for its high specificity and purity in protein purification. Anecdotally, it is commonly used as a follow-on or alternative to His tag when high purity is desired.
