"TriAltus Bioscience: The Intersection of Name, Innovation, and Excellence" is an article that delves into the meaning behind the TriAltus name and the company's journey with the innovative CLīM technology. It aims to inform and inspire readers about the advancements and commitments of TriAltus in the field of protein purification.
"Unlocking the Magic of CL7/Im7 Protein Purification: A Game-Changer for Scientists" is an article designed to inspire and inform fellow protein scientists about the groundbreaking CL7/Im7 protein purification system.
Choosing the right reagents is crucial when preparing to conduct affinity protein purification. After you’ve chosen a tag system to use, the next step is to choose an appropriate resin. This blog post will outline some of the differences between 4B and 6B agarose resin and how to choose the right resin for your needs.
Different cell expression systems offer certain benefits and drawbacks to yield and post-translational modification. TriAltus has expanded our plasmid offerings to include BacMam vectors so that the CL7/Im7 affinity purification system can be used in a wider scope of applications.
Endotoxin testing is an important standard of many proteins’ quality control reporting. Verifying the amount of endotoxin in a sample of protein is critical to ensure that it is safe for research and therapeutic applications.
Eluting your protein of interest is the final step in affinity chromatography before obtaining a pure, useful sample for further study. Common methods include elution using proteolytic elution, low pH elution, and denaturant elution. An alternative to these options is what’s known as Gentle Elution Buffer, or 3.6 M MgCl2 pH 6.6.
Based on the natural affinity between biotin and streptavidin, Strep-tag is one of the most specific-binding speciality tags on the market. Its high specificity positions it as an alternative or supplement to His-trap, which is based on a non-specific binding of His to metal ions in resin.
Human growth hormone (hGH), also known as somatropin, is a hormone secreted by the pituitary gland. This blog post gives an overview of the uses and features of hGH, as well as its optimized soluble purification using the CL7/Im7 system.
Improvements on the methods and manner of delivery of ribonucleoprotein (RNP) complexes have been made in order to improve efficiency and minimize off-target effects. This blog post reviews the current state of RNP complex delivery systems.
Low protein yield is a common issue in protein purification, especially when it comes to isolating large or complex structures. In lieu of using denaturants to elute protein from an affinity column, protease elution can be a welcome alternative to give tag-free protein.