Discover how TriAltus achieved >99% purity of multi-subunit RNA polymerases using a single-step CL7/Im7 system—no polishing steps, no compromise. Perfect for structural biology, CRISPR, and high-throughput assays.
Different cell expression systems offer certain benefits and drawbacks to yield and post-translational modification. TriAltus has expanded our plasmid offerings to include BacMam vectors so that the CL7/Im7 affinity purification system can be used in a wider scope of applications.
Multi-subunit proteins present roadblocks because of their size and the expression of multiple units. In this blog post, we discuss the one-step purification of ttRNAP and mtRNAP, two bacterial RNA polymerases, using the CL7/Im7 system.
Protein fusion tags make affinity chromatography possible for many proteins, particularly those for which no affinity-quality antibody is available. In addition to improving conditions for purification, tags can facilitate detection and protein interaction studies.
