Unlocking the Magic of CL7/Im7, or "CLīM", Protein Purification: A Game-Changer for Scientists

Introduction to CLīM Protein Purification: The Rising Star in Protein Science

Welcome to the exciting world of protein purification! Today, we're going to explore the transformative power of the CLīM protein purification system. This groundbreaking technology is reshaping our approach to protein science, making purification processes more efficient, pure, and user-friendly. So, buckle up, scientists, as we embark on a journey through this revolutionary system!

The Science Behind CLīM: Understanding the CL7/Im7 Affinity Complex

At the heart of the CLīM system lies the extremely high-affinity interaction between two proteins: the CL7 tag and the small and stable immunity protein 7 (Im7) ligand. The CL7 tag is an ingeniously engineered variant of a bacterial toxin CE7 that was designed to retain ultra high affinity to the specific CE7 inhibitor, Im7, while eliminating any toxic activities. The Im7 ligand is used to make a robust specialty resin through efficient, directed coupling of the Im7 to agarose resin. Because of the extraordinary affinity, this interaction is impervious to high salt (up to 3M) and can be used at any pH between 4-10.  This high-affinity pair is what makes the CLīM system an exceptional tool in our purification toolkit.

 

Image of CL7 bound to Im7 Resin Ligand

Revolutionizing Protein Purification: Why CLīM Stands Out

The CLīM system isn't just another method; it's a leap forward in protein purification technology. With its superior binding capacity, extraordinary stability and reusability, it stands out as a robust and economical choice for scientists. CLīM also stands out because of its high purity in a single chromatographic step, ensuring ultra high purity of the final product, and offering cost and time savings by eliminating the need for additional cleanup steps. It streamlines the purification process, delivering pure proteins efficiently and cost-effectively, making it the top value choice for scientists seeking simplicity and excellence in their research endeavors.

 

 

Maximizing Laboratory Efficiency: High Column Capacities and Its Impact

One of the most striking advantages of the CLīM system is its impressive binding capacity, capable of handling up to 60 mg/mL. This high capacity is not just a number; it represents a significant enhancement in purification efficiency, reducing both time and cost in your laboratory operations.

Enhancing Protein Expression and Purity: The Role of CL7 Tag in Protein Expression and Solubility

But the CL7 tag isn't all about purification. It also plays a pivotal role in boosting protein expression levels and enhancing solubility. This multifunctional aspect of the CL7 tag is particularly beneficial for complex target proteins, ensuring successful outcomes in a variety of research scenarios.

Adapting to Diverse Protein Challenges: Versatility in Purifying Complex Proteins

The CLīM system shines when it comes to versatility. Whether you're dealing with membrane proteins, DNA binding proteins, multisubunit complexes, or other challenging targets, CLīM adapts effortlessly, offering high-purity results even in high-salt conditions. This adaptability is a testament to the system's innovative design.

The Future of Protein Purification: Innovations and Potential in Protein Science

As we look to the future, the CLīM system represents a beacon of innovation in protein purification. Its potential to drive further advancements in the field is boundless, paving the way for new discoveries and developments in protein science.

FAQs About CLīM Protein Purification

In this section, we'll address some of the most common inquiries about the CL7/Im7 system, providing you with deeper insights into its capabilities and applications.

 

  1. Is the CLiM Affinity Tag System compatible with E. coli and mammalian expression systems? Absolutely! Our CLiM Affinity Tag System is versatile and works seamlessly with both E. coli and mammalian expression systems, ensuring flexibility and adaptability in your research.
  2. Where is the tag positioned: N-terminus or C-terminus? The beauty of the CL7 tag lies in its flexibility. It can be adeptly fused either to the N- or C-terminus of your protein. We offer a range of vectors tailored to the specific needs of your protein, ensuring optimal results.
  3. Which protease do you use for elution? For elution, we utilize either PreScission protease or SUMO protease, depending on what best suits your protein's characteristics and your purification goals.  These enzymes are cysteine proteases, which have no reported off-target cleavage, in stark contrast to serine/threonine proteases like thrombin or Factor Xa.
  1. What tag is on SUMO and PreScission protease? Our SUMO protease comes equipped with a His-tag, while the PreScission protease features a glutathione S-transferase (GST) tag, each designed to enhance purification efficiency by allowing capture upon proteolytic elution using a tandem nickel or glutathione column.
  2. Which plasmid should I use? Choosing the right plasmid is crucial, and we've got you covered. Visit our comprehensive Plasmid Guide for detailed information to help you select the most suitable plasmid for your unique protein purification needs.
  3. Can you regenerate the resin? How many times? Yes, the Im7 resin's durability is remarkable. It can be regenerated with 6M guanidine and reused numerous times without any significant loss of binding capacity, offering you both reliability and cost-effectiveness.
  4. Are kits available for first-time users of the CL7 tag? Definitely! We offer starter kits specially designed for first-time users. These kits provide everything you need to get started with the CL7 tag system. Check them out here: https://trialtusbioscience.com/collections/kits-and-buffers.
  5. What conditions do you recommend for proper column storage? For optimal column storage, we recommend a solution of 20 mM Tris pH 8.0, 0.5M NaCl, and 5% glycerol. For long-term storage, adding sodium azide at a 0.05% final concentration is advisable to prevent microbial contamination.
  6. What happens to the resin if it is stored at -20°C? Storing the resin at -20°C is not recommended. Such freezing temperatures can adversely affect the resin's quality and performance.
  7. Do you do custom protein purification? Yes, indeed! We offer custom protein purification services. Please contact our technical services at info@trialtusbioscience.com for a personalized consultation to discuss your specific needs and how we can assist you.

 

Embracing the New Era in Protein Purification

The CLīM  protein purification system is more than just a technological advancement; it's a new era in protein science. As we continue to explore and harness its capabilities, we invite you to join us in this exciting journey. Together, let's unlock the full potential of protein research and pave the way for scientific breakthroughs!

 

Learn more about CL7/Im7 here.  


Contact us for inquiries and collaboration opportunities. info@trialtusbioscience.com

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