Overview

TriAltus’ Tn5 Transposase is a recombinant enzyme used in DNA processing workflows where controlled fragmentation and adapter incorporation are commonly required. As a transposase, Tn5 is frequently selected during method development for applications that benefit from streamlined DNA preparation and consistent enzymatic handling. This offering is intended for research workflows and is supplied as transposase-only (not pre-loaded) in a format.

Also known as: Tn5 transposase, recombinant Tn5, hyperactive Tn5 transposase, transposase enzyme (Tn5)

Why Performance and Consistency Matter for Tn5 Transposase

Transposase-enabled workflows are sensitive to enzyme quality and lot-to-lot consistency because small differences in enzyme preparation can shift reaction behavior, library profiles, and downstream comparability. Consistent Tn5 transposase supports more reproducible outcomes across runs and helps reduce variability during assay optimization and standardized processing workflows.

Bulk Supply & Program Support

TriAltus supports bulk supply and tailored formats for organizations requiring dependable, long-term access to critical enzymes. Production under ISO13485–compliant processes and the CLīM™ purification platform enable batch consistency and efficient cost-of-goods at scale, supporting volume-based pricing for sustained programs. Contact TriAltus to discuss volume, documentation, packaging, and supply planning.

Key Benefits

• Recombinant Tn5 transposase for research DNA processing workflows
  

• Suitable for method development where controlled reaction behavior is important

• Manufactured using TriAltus CLīM™ purification platform for high-purity preparations
  

Specifications

PURITY | ≥80%

ENZYME SOURCE | Tn5 transposase expressed in E. coli

STORAGE/SHIPPING CONCENTRATION | 1.0 mg/mL

FORMAT | Transposase-only (not pre-loaded / not complexed)

STORAGE BUFFER | 10 mM Tris pH 8, 125 mM NaCl, 0.5 mM TCEP, 50% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

Produced under ISO 13485–compliant processes.

Tn5 Transposase Data Sheet

SKU List

100 ug: 40-3420 | 500 ug: 40-3425 | 1 mg: 40-3430 | 5 mg: 40-3435 | 10 mg: 40-3440 | Bulk: Inquire

Applications

DNA fragmentation and preparation workflows (method development)

Tn5 transposase is commonly used in workflows where DNA fragmentation and streamlined preparation steps are evaluated and optimized for downstream analysis.

Library preparation workflow development

Often used during development and optimization of library preparation methods where consistent enzymatic behavior supports comparability across conditions.

Transposase-based assay optimization

Useful for teams developing transposase-enabled assays and refining reaction inputs to achieve consistent processing profiles.

Genomic DNA processing studies

Applied in research workflows involving genomic DNA handling where reproducible processing is important for downstream measurement and analysis.

Learn More

• Learn how TriAltus achieves high purity using CLīM™ purification.

• Explore peer-reviewed publications describing the underlying protein production platform.

• View the the Purified Phi29 DNA Polymerase page for comparison with other molecular biology enzymes.

FAQs

Is this Tn5 supplied pre-loaded with adapters?

No. This product is supplied as transposase-only (not pre-loaded / not complexed). It’s intended for workflows where you prepare or source adapters separately and optimize loading/assembly conditions as needed.

What is this Tn5 transposase typically used for in research workflows?

Tn5 is commonly used in transposase-enabled DNA processing workflows where controlled fragmentation and streamlined preparation steps are evaluated during method development and protocol optimization.

Why does lot-to-lot consistency matter for Tn5-based workflows?

Transposase reactions can be sensitive to enzyme preparation and handling. Lot consistency helps keep reaction behavior more comparable across runs, which is especially useful when you’re optimizing conditions or running standardized protocols over time.

Can I use this for library prep method development without committing to a fixed kit format?

Yes. Because it’s transposase-only, it fits workflows where teams want flexibility to tune adapter design, loading ratios, and reaction conditions during development.

Do you support bulk supply for ongoing programs?

Yes. TriAltus supports bulk supply and can provide quotes based on volume, preferred formats, and documentation needs for sustained programs.