TriAltus' highly pure, tag-free Phi29 DNA polymerase is a highly processive, isothermal enzyme from Bacillus subtilis phage phi29, known for its exceptional strand displacement and ability to synthesize long DNA fragments up to 70 kb. With a robust 3'→5' proofreading exonuclease function, it ensures high-fidelity amplification, making it ideal for applications like whole genome amplification and DNA sequencing preparation.

SPECIFICATIONS

PURITY | ≥95%

ENZYME SOURCE | Phi29 DNA polymerase (from Bacillus subtilis phage phi29) is expressed in E. coli and is purified with our CLīM technology.

STORAGE/SHIPPING CONCENTRATION | 10 U/µL

STORAGE BUFFER | 10 mM Tris-Cl pH 7.4, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween® 20, 0.5% IGEPAL® CA-630, 50% glycerol

RECOMMENDED STORAGE CONDITIONS | -20°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

SKU | 

3,000 Units: 40-2414

10,000 Units: 40-2420

Customize concentration and scale to meet your specific research needs. Please Inquire.

One unit is defined as the amount of enzyme required to convert 0.5 pmol dNTPs to acid-insoluble material in 10 min at 30°C.

Phi29 DNA Polymerase Data Sheet

Use of TriAltus products falls under our limited license agreement and is for non-profit research use only. For commercial use, please contact us for licensing.