Overview

TriAltus provides purified Streptococcus pyogenes Cas9 (SpCas9) nuclease for CRISPR genome editing workflows. This enzyme is supplied tag-free and formulated for straightforward complexing with guide RNA to generate Cas9–gRNA ribonucleoprotein (RNP) for editing applications.

Also known as: SpCas9 nuclease, CRISPR Cas9 protein, Streptococcus pyogenes Cas9, Cas9 CRISPR nuclease

Why purity matters for CRISPR editing

Cas9 performance is highly sensitive to impurities that can interfere with RNP assembly, increase background activity in downstream assays, or introduce variability across experiments. For that reason, this SpCas9 is manufactured and released with ~99% purity by SDS-PAGE and endotoxin <0.1 EU/µg measured by Limulus Amebocyte Lysate (LAL) assay, supporting reproducible results in research workflows that are sensitive to endotoxin and lot-to-lot variation.

Bulk Supply & Program Support

TriAltus supports bulk supply and tailored formats for organizations requiring dependable, long-term access to critical enzymes. Production under ISO 13485–compliant processes and the CLīM™ purification platform enable batch consistency and efficient cost-of-goods at scale, supporting volume-based pricing for sustained programs. Contact TriAltus to discuss volume, documentation, packaging, and supply planning.

Key Benefits

• Tag-free SpCas9 for genome editing workflows

• Dual NLS to support nuclear targeting (when used in appropriate delivery formats)

• ~99% purity (SDS-PAGE)

• Low endotoxin (<0.1 EU/µg, LAL assay)

• Clear release specs: storage buffer, concentration

Specifications

PURITY | ~99% (SDS-PAGE gel analysis)

ENZYME SOURCE | Streptococcus pyogenes Cas9 is expressed in E. coli with two (N-terminal and C-terminal) nuclear localization signals (NLS), and is purified with our CL7 tag technology. 

ENDOTOXIN | <0.1 EU/ug by LAL assay

STORAGE/SHIPPING CONCENTRATION | 5 mg/mL

STORAGE BUFFER | 10 mM Tris-Cl pH 8.0, 250 mM NaCl, 50% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

Produced under ISO 13485–compliant processes.

Cas9 Data Sheet

SKU List

500 µg: 40-1325 | 1 mg: 40-1330 | Bulk: Inquire

Applications

Purified SpCas9 protein from TriAltus is designed for CRISPR genome editing research workflows that require high purity, low endotoxin, and consistent performance.

CRISPR–Cas9 RNP Genome Editing

This product is commonly used for complexing with guide RNA to form Cas9–gRNA ribonucleoprotein (RNP) for genome editing studies. RNP-based approaches enable transient Cas9 activity and are widely applied in workflows where controlled editing and reduced background exposure are important.

Cell-Based Genome Editing Research

Low endotoxin levels and defined purity specifications support use in cell-based CRISPR research, particularly in assays sensitive to endotoxin-related variability. Applications include optimization of genome editing conditions and comparative evaluation of guide RNA designs.

Method Development and Assay Optimization

Purified SpCas9 is frequently used during CRISPR method development, including:

• Guide RNA screening and validation

• RNP assembly and stability optimization

• Evaluation of delivery and experimental conditions

• Benchmarking editing performance across research systems

In Vitro CRISPR Activity Studies

This product is suitable for in vitro CRISPR studies, such as DNA cleavage assays and biochemical characterization of Cas9 activity, where defined protein quality and reproducibility are essential.

For research use only. Not for therapeutic use.

Learn More

• Learn how TriAltus achieves high purity with one-step CLīM™ purification.

• See peer-reviewed publications supporting the platform.

FAQs

Is this SpCas9 protein tag-free?

Yes. The Cas9 protein is supplied without affinity tags, which helps minimize interference during RNP assembly and downstream genome editing experiments.

What purity and endotoxin levels are provided?

Each production lot meets release specifications of ~99% purity by SDS-PAGE and endotoxin <0.1 EU/µg, determined using the LAL assay, to support reproducible performance in endotoxin-sensitive applications.

Is this product suitable for CRISPR RNP genome editing?

Yes. The protein is designed for efficient complexing with guide RNA to form Cas9–gRNA ribonucleoprotein (RNP) for genome editing research applications.

Does the protein include nuclear localization signals (NLS)?

Yes. The Cas9 protein contains dual nuclear localization signals, which support nuclear targeting when used in appropriate delivery formats.