The PreScission protease (PSC) recognizes and cleaves the sequence LEVLFQ/GP (where “/” indicates the cleavage site) to release the target protein from the Im7-bound CL7 tag.  

SPECIFICATIONS

PURITY | >99% (SDS gel analysis)

ENZYME SOURCE | Human Rhinovirus (HRV) 3C Protease overexpressed in E. coli with a glutathione S-transferase (GST) tag

CLEAVAGE SITE | PSC recognizes the sequence LEVLFQGP and cleaves between the glutamine (Q) and glycine (G)

CLEAVAGE EFFICIENCY |  20 ug protease will digest 2 mg protein with 99% efficiency at 4°C in 60 minutes.

STORAGE/SHIPPING CONCENTRATION | ~5 mg/mL

SHIPPING CONDITION | We ship PreScission Protease on dry ice in buffer (20 mM Tris (pH 8.0), 0.1 M NaCl, 1 mM EDTA, 50% glycerol, 1 mM βMe).

RECOMMENDED STORAGE CONDITIONS | -80°C long term or -20°C for 2-3 months in shipping buffer

EXPIRATION | 6 months from receipt when stored as directed

SKU | 

1 mg: 30-2130

5 mg: 30-2135

10 mg: 30-2140

50 mg: 30-2146

100 mg: 30-2150

Additional processing information: After a CL7-tagged protein binds the activated Im7 resin, PSC is applied to the column to release the protein from the CL7 tag. The final protease concentration in the eluted sample comprises ~1.5-3% of total impurities. If needed, PSC can be purified from the sample with a GST-trap column or size exclusion chromatography.

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