Overview

SUMO Protease (ULP1) is a site-specific protease used to remove SUMO fusion tags from recombinant proteins. It cleaves after the C-terminal glycine at the end of the SUMO sequence, enabling tag removal that can yield the native target sequence without additional amino acids when the fusion is designed at the SUMO C-terminus.

Also known as: SUMO protease, ULP1 protease, Ulp1, SUMO-specific protease

Why Purity and Specificity Matter for SUMO Protease

Tag removal workflows are sensitive to protease purity and specificity because contaminating proteases can introduce off-target cleavage and reduce recovery of the desired protein. A high-purity SUMO protease supports reproducible digestion conditions and predictable tag removal—especially for programs standardizing purification workflows across multiple constructs and lots.

Bulk Supply & Program Support

TriAltus supports bulk supply and tailored formats for organizations that require dependable, long-term access to SUMO protease for standardized purification workflows. Volume-based pricing is available for program-scale demand and cost-of-goods targets. Production is performed under ISO 13485–compliant processes. Contact TriAltus to discuss volume requirements, documentation needs, packaging preferences, and to request a quote.

Key Benefits

• Scarless cleavage design: cleaves after SUMO C-terminal glycine to yield native sequence (construct-dependent)

• High purity: ~100% purity by SDS gel analysis

• Efficient digestion at 4°C: 1 µg digests 2 mg protein with 99% efficiency in 40 minutes
  

Specifications

PURITY | ~100% (SDS gel analysis)

ENZYME SOURCE | Yeast protein overexpressed in E. coli with histidine (His) tag

CLEAVAGE SITE | SUMO Protease (ULP1) cleaves after the C-terminal glycine at the end of the SUMO sequence. If the target protein is fused downstream of the SUMO C-terminus, SUMOP cleavage renders the native protein sequence without any additional amino acids. 

CLEAVAGE EFFICIENCY |  1 ug protease will digest 2 mg protein with 99% efficiency at 4°C in 40 minutes.

STORAGE/SHIPPING CONCENTRATION | ~7.5 mg/mL

SHIPPING CONDITIONS | We ship SUMO protease on dry ice in buffer (15 mM Tris (pH 8.0), 0.2 M NaCl, 50% glycerol). 

RECOMMENDED STORAGE CONDITIONS | -80°C long term or -20°C for 2-3 months in shipping buffer

EXPIRATION | 6 months from receipt when stored as directed

Produced under ISO 13485–compliant processes.

SKU List

1 mg: 30-1130 | 5 mg: 30-1135 | 10 mg: 30-1140 | 50 mg: 30-1146 | 100 mg: 30-1150 | Bulk: Inquire

Applications

Scarless SUMO tag removal

ULP1 cleaves after the SUMO C-terminal glycine; when the target is fused directly downstream, cleavage can yield the native sequence without extra residues.

Low-temperature digestion

A representative condition is 99% digestion at 4°C in 40 minutes (1 µg protease per 2 mg target protein), supporting workflows that benefit from gentle handling.

Standardized purification programs

Defined digestion conditions and high purity support protocol consistency across multiple constructs and repeated production runs.

Learn More

• Compare alternative tag cleavage options on the PreScission Protease page
  

• Review high-purity genome editing enzymes on the Purified CRISPR-Cas9 Protein page

FAQs 

Does SUMO protease cleavage leave extra residues?

When the target protein is fused directly downstream of the SUMO C-terminus, ULP1 cleavage after the C-terminal glycine can yield the native target sequence without additional amino acids (construct-dependent).

What are typical starting digestion conditions?

A representative condition is 1 µg protease per 2 mg target protein for 99% efficiency at 4°C in 40 minutes, then optimize ratio/time as needed.

Does the SUMO protease have a tag?

This SUMO protease is overexpressed in E. coli with a histidine (His) tag.

Are other tag options available?

Yes. Alternative tag formats or tailored configurations may be available depending on program needs and downstream requirements. Contact TriAltus to discuss your preferred tag format.