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Sortase A, Heptamutant (Tag-Free)

Product

Sortase A, Heptamutant (Tag-Free)

$375

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Quantity

Buy in Bulk

Quantity

Buy in Bulk

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This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.

SPECIFICATIONS

PURITY | ≥95%

ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.

STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL

STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

SKU | Tag Free:

100 µg: 40-2320

500 µg: 40-2325

1 mg: 40-2330

This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.

SPECIFICATIONS

PURITY | ≥95%

ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.

STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL

STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

SKU | Tag Free:

100 µg: 40-2320

500 µg: 40-2325

1 mg: 40-2330

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40-2414, 40-2420

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40-1212, 40-1220, 40-1225

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40-1320, 40-1325

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Specifications

Data Sheets

Similar Products

Shipping

Buy Now

This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.

SPECIFICATIONS

PURITY | ≥95%

ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.

STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL

STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

SKU | Tag Free:

100 µg: 40-2320

500 µg: 40-2325

1 mg: 40-2330