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Product
Sortase A, Heptamutant (Tag-Free)
$375
Quantity
Buy in Bulk
Quantity
Buy in Bulk
Quantity
Buy in Bulk
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This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.
SPECIFICATIONS
PURITY | ≥95%
ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.
STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL
STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol
RECOMMENDED STORAGE CONDITIONS | -80°C
EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.
SKU | Tag Free:
100 µg: 40-2320
500 µg: 40-2325
1 mg: 40-2330
This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.
SPECIFICATIONS
PURITY | ≥95%
ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.
STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL
STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol
RECOMMENDED STORAGE CONDITIONS | -80°C
EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.
SKU | Tag Free:
100 µg: 40-2320
500 µg: 40-2325
1 mg: 40-2330



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Specifications
Data Sheets
Similar Products
Shipping

Buy Now
This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.
SPECIFICATIONS
PURITY | ≥95%
ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.
STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL
STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol
RECOMMENDED STORAGE CONDITIONS | -80°C
EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.
SKU | Tag Free:
100 µg: 40-2320
500 µg: 40-2325
1 mg: 40-2330






