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Sortase A, Heptamutant (His-tagged)

Product

Sortase A, Heptamutant (His-tagged)

$375

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Quantity

Buy in Bulk

Quantity

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TriAltus offers a high performance N-terminal 6x His-tagged Sortase A, Heptamutant. This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.

SPECIFICATIONS

PURITY | ≥95%

ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.

STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL

STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

SKU | 6x His-tagged:

100 µg: 40-2520

500 µg: 40-2525

1 mg: 40-2530

TriAltus offers a high performance N-terminal 6x His-tagged Sortase A, Heptamutant. This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.

SPECIFICATIONS

PURITY | ≥95%

ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.

STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL

STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

SKU | 6x His-tagged:

100 µg: 40-2520

500 µg: 40-2525

1 mg: 40-2530

Phi29 DNA Polymerase Data Sheet

40-2414, 40-2420

AapCas12b Protein Data Sheet

40-1212, 40-1220, 40-1225

Cas9 Data Sheet

40-1320, 40-1325

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Specifications

Data Sheets

Similar Products

Shipping

Buy Now

TriAltus offers a high performance N-terminal 6x His-tagged Sortase A, Heptamutant. This engineered enzyme from Staphylococcus aureus (mutations P94R, D160N, D165A, Y187L, E189R, K190E, K196T) enables site-specific protein modification and ligation with exceptional precision. The heptamutant variant of Sortase A is calcium-independent, making it effective in both intracellular and extracellular environments. Applications include site-specific protein labeling, antibody modification, protein circularization, and live-cell surface conjugation with molecules such as fluorescent dyes, biotin, and nucleic acids.

SPECIFICATIONS

PURITY | ≥95%

ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli and is purified with our CLīM technology.

STORAGE/SHIPPING CONCENTRATION | ~ 1 mg/mL

STORAGE BUFFER | 20 mM Tris HCl pH 8.0, 0.5 M NaCl, 20% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

SKU | 6x His-tagged:

100 µg: 40-2520

500 µg: 40-2525

1 mg: 40-2530