Overview

TriAltus’ purified AapCas12b protein is a tag-free CRISPR Type V-B nuclease designed for genome editing research requiring high specificity and alternative PAM recognition. Derived from Alicyclobacillus acidiphilus, AapCas12b offers distinct biochemical and targeting characteristics that make it well suited for applications where nuclease selectivity and controlled activity are important.

Also known as: AapCas12b nuclease, CRISPR Cas12b protein, Cas12b CRISPR nuclease, Alicyclobacillus Cas12b

Why Purity and Activity Matter for Cas12b

Cas12b nucleases differ from Cas9 and Cas12a in both structural and biochemical properties, placing added emphasis on protein purity and consistent activity. Cas12b enzymes are often selected for their distinct PAM requirements and higher targeting stringency, making inactive protein populations or impurities more likely to impact cleavage efficiency and reproducibility. High-purity, well-characterized Cas12b supports predictable RNP formation and controlled nuclease activity, which is particularly important when comparing editing outcomes across experiments or evaluating alternative CRISPR systems.

Bulk Supply & Program Support

TriAltus supports bulk supply and tailored formats for organizations requiring dependable, long-term access to critical enzymes. Production under ISO 13485–compliant processes and the CLīM™ purification platform enable batch consistency and efficient cost-of-goods at scale, supporting volume-based pricing for sustained programs. Contact TriAltus to discuss volume, documentation, packaging, and supply planning.

Key Benefits

• Tag-free AapCas12b for streamlined RNP assembly

• CRISPR Type V-B nuclease with alternative PAM recognition

• Dual nuclear localization signals (NLS) to support nuclear targeting

• Suitable for precision-focused genome editing workflows

Specifications

PURITY | ≥90%

ENZYME SOURCE | Alicyclobacillus acidiphilus Cas12b is expressed in E. coli with two C-terminal nuclear localization signals (NLS) and is purified with our CLīM technology.

STORAGE/SHIPPING CONCENTRATION | ~ 5 mg/mL

STORAGE BUFFER | 10 mM Tris-Cl pH 8.0, 250 mM NaCl, 50% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. 

Produced under ISO 13485–compliant processes.

AapCas12b Data Sheet

SKU List

20 µg: 40-1212 | 100 µg: 40-1220 | 500 µg: 40-1225 | Bulk: Inquire

Applications

CRISPR–Cas12b RNP Genome Editing

AapCas12b can be complexed with guide RNA to form RNPs for genome editing research, offering an alternative to Cas9 and Cas12a in workflows that benefit from distinct PAM recognition and nuclease behavior.

Precision-Focused Editing Studies

Researchers use Cas12b to explore genome editing strategies where specificity and controlled activity are priorities, including comparative studies across CRISPR systems.

In Vitro Cleavage and Method Development

Purified AapCas12b is suitable for in vitro DNA cleavage assays and workflow development where defined protein quality supports reproducible results.

Learn More

• Learn how TriAltus achieves high purity using one-step CLīM™ purification.

• Explore peer-reviewed publications describing the underlying purification platform.

• Compare CRISPR nuclease options with the Purified CRISPR-Cas9 Protein page.

• View the LbCas12a Protein page for additional Cas12 family enzymes.

FAQs

Is AapCas12b supplied tag-free?

Yes. The protein is supplied without affinity tags to support clean RNP formation.

What CRISPR type is AapCas12b?

AapCas12b is a Class 2, Type V-B CRISPR nuclease.

Is this suitable for genome editing research?

Yes. AapCas12b is intended for research-use genome editing and comparative CRISPR studies.