plasmid 31 option 1
plasmid #31 option 2
plasmid #31 option 3
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Plasmid #31 – DISCONTINUED

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Plasmid #31 enables the labeling of target proteins with both cleavable N-terminal His8 and cleavable C-terminal CL7 tags. 

SKU: 20-1031

EXPRESSION
Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli.

PEPTIDE TAG
The C-terminal CL7 tag is downstream of three PreScission protease (PSC) and one Sortase A (SRT) cleavage site. 

CLEAVAGE SITE(S)
Plasmid #31 includes a C-terminal SUMO cleavage site, upstream of the His8 tag; a Tobacco Etch Virus (TEV) cleavage site between the His8 tag and target protein; a SRT site downstream of the target protein; and three PSC cleavage sites between the SRT and CL7 tag. 

OTHER TAGS
This plasmid includes N-terminal Trx and His8 tags. 

CLONING OPTIONS
1. HindIII/SpeI Insertion Site – Trx | SUMO | His8 | TEV | Gene of Interest | SRT | PSC | PSC | PSC | CL7

2. KpnI/SpeI Insertion Site – Trx | SUMO | His8 | Gene of Interest | SRT | PSC | PSC | PSC | CL7

3. Bsu36I/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | CL7

    This insertion option maintains the Gene of Interest’s wildtype sequence, without adding any extra residues. The N-terminus of the Gene of Interest must include the following to complete the SUMO C-terminal sequence:

    EXPIRATION | 6 months from receipt when stored as directed

    Plasmid Sequences are available on the Plasmid Guide Page

    Use of TriAltus products falls under our limited license agreement and is for non-profit research use only. For commercial use, please contact us for licensing.