Plasmid #8 is used for SUMO protease (SUMOP) purification.
Approximately 0.2-0.3 mg purified SUMOP (expressed with Plasmid #7) cleaves 35-40 mg of on-column target protein in ~1.5 hours.
Transcription is driven by the T7 RNA polymerase, and the plasmid is designed for expression in E. Coli.
SUMOP can be purified via the N-terminal Trx or C-terminal His tag.
A SUMO protease cleavage site is located upstream of the SUMOP coding region.
An N-terminal Trx region begins the coding region and a C-terminal His tag terminates it.