Plasmid #33 enables target proteins to be labeled with C-terminal His8 and CL7.
Transcription is driven by the T7 RNA polymerase, and the plasmid is designed for expression in E. Coli.
The C-terminal CL7 tag is downstream of three PreScission (PSC) cleavage sites, one Sortase A (SRT) cleavage site, and a His8 tag.
Plasmid #33 includes a N-terminal SUMO cleavage site and three PSC and one SRT C-terminal cleavage sites.
The encoded region begins with a Trx tag, and a C-terminal His8 tag is also present upstream of the CL7 tag.