Plasmid #34 enables target proteins to be labeled with C-terminal His8 and CL7. Protease cleavage removes both tags.
SKU: 20-1033
EXPRESSION
Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli.
PEPTIDE TAG
The C-terminal CL7 tag is downstream of Sortase A (SRT) and PreScission protease (PSC) cleavage sites and a His8 tag.
CLEAVAGE SITE(S)
N-terminal SUMO and C-terminal SRT and PSC cleavage sites are available in Plasmid #34.
OTHER TAGS
The encoded region begins with a Trx tag and includes a C-terminal His8 tag upstream of CL7.
CLONING OPTIONS
1. HindIII/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7
This insertion option results in 7 extra aa at the N terminus of the target following SUMO protease cleavage.
2. KpnI/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7
This insertion option results in 2 extra aa at the N terminus of the target following SUMO protease cleavage.
3. Bsu36I/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7
This insertion option maintains the Gene of Interest’s wildtype sequence, without adding any extra residues. The N-terminus of the Gene of Interest must include the following to complete the SUMO C-terminal sequence:
EXPIRATION | 6 months from receipt when stored as directed
Plasmid Sequences are available on the Plasmid Guide Page
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