Plasmid #33 enables target proteins to be labeled with C-terminal His8 and CL7. The His8 tag remains attached depending on the protease utilized.
SKU: 20-1033
EXPRESSION
Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli.
PEPTIDE TAG
The C-terminal CL7 tag is downstream of three PreScission protease (PSC) cleavage sites, one Sortase A (SRT) cleavage site, and a His8 tag.
CLEAVAGE SITE(S)
Plasmid #33 includes an N-terminal SUMO cleavage site, three PSC, and one SRT C-terminal cleavage sites.
OTHER TAGS
The encoded region begins with a Trx tag. A C-terminal His8 tag is also present upstream of the CL7 tag.
CLONING OPTIONS
1. HindIII/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | His8 | PSC | PSC | PSC | CL7
This insertion option results in 7 extra aa at the N terminus of the target following SUMO protease cleavage.
2. KpnI/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | His8 | PSC | PSC | PSC | CL7
3. Bsu36I/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | His8 | PSC | PSC | PSC | CL7
This insertion option maintains the Gene of Interest’s wildtype sequence, without adding any extra residues. The N-terminus of the Gene of Interest must include the following to complete the SUMO C-terminal sequence:
EXPIRATION | 6 months from receipt when stored as directed
Plasmid Sequences are available on the Plasmid Guide Page
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