FPLC columns are packed with our Activated Im7 Resin for use with proteins tagged with CL7. Columns are available in 1 ml and 5 ml sizes.
Capable of up to 100 reactivations, TriAltus’ Activated Im7 Resin retains a 1:1 molar binding ratio during repeated uses. The Activated Im7 Resin has been used to purify a variety of proteins – including membrane and multi-subunit proteins.
SIMPLICITY – In a single purification step, this protocol yields purified proteins that exhibit high yield, high purity, and a high activity level.
REUSABILITY – The Im7 resin is reusable with no observable loss in capacity.
RESILIENCY – The Im7 Resin retains a 1:1 molar binding ratio after multiple reactivations in a variety of conditions, including various salt and detergent concentrations.
SPECIFICATIONS
PARTICLE SIZE | Crosslinked agarose 6B beads (45 – 165 μM)
PH STABILITY | The Im7 protein is stable on the beads at pH 3-10. However, CL7/Im7 binding is stable at pH 4.2 – 10 only.
SALT STABILITY | ≤ 4M NaCl tested
BINDING CAPACITY | 35-40 mg CL7/mL resin
STORAGE/SHIPPING CONCENTRATION | 50/50 buffer/resin slurry. Available in 1-mL and 5-mL.
RECOMMENDED OPERATING TEMPERATURE | 4°C or room temperature
REACTIVATION DETAILS | To remove the CL7 protein and reactivate the resin, wash the column with Gdn-HCl, exchanging it into physiological buffer.
Additional information: Target protein characteristics (e.g., protein size, conformation, and concentration); flow rate (i.e., lower flow rates may increase the binding capacity); and other parameters (e.g., pH and temperature) can affect the column binding capacity.