BacMam NT His8-CL7-eGFP enables target proteins to be labeled with N-terminal His8, CL7, and eGFP tags. Protease cleavage removes all tags.
The plasmid is designed for transient expression in mammalian cells or for baculovirus transduction of mammalian cells (BacMam). Expression is constitutive and driven by the CMV promoter; a Kozak sequence is immediately upstream of the start codon.
The N-terminal CL7 tag is between the His8 and eGFP tags, and a PreScission protease (PSC) cleavage site is C-terminal to all tags.
C-terminal PSC P
His8 tag is N-terminal, followed by CL7, then eGFP.
VOLUME | 10 ug at 500 ng/uL
EXPIRATION | 12 months from receipt when stored as directed
Plasmid Sequences are available on the Plasmid Guide Page
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