BacMam CT eGFP-CL7-H8 enables target proteins to be labeled with C-terminal eGFP, CL7, and His8 tags. Protease cleavage removes all tags.
The plasmid is designed for transient expression in mammalian cells or for baculovirus transduction of mammalian cells (BacMam). Expression is constitutive and driven by the CMV promoter. A Kozak sequence should be added to the 5’ end of the gene insert for efficient translation.
The C-terminal CL7 tag is between the eGFP and His8 tags, and a PreScission protease (PSC) cleavage site is N-terminal to all tags.
N-terminal PSC P
eGFP tag is N-terminal, followed by CL7, then His8.
VOLUME | 10 ug at 500 ng/uL
EXPIRATION | 6 months from receipt when stored as directed
Plasmid Sequences are available on the Plasmid Guide Page