BacMam NT His8-CL7-eGFP enables target proteins to be labeled with N-terminal His8, CL7, and eGFP tags. Protease cleavage removes all tags.
SKU: 20-4000
EXPRESSION
The plasmid is designed for transient expression in mammalian cells or for baculovirus transduction of mammalian cells (BacMam). Expression is constitutive and driven by the CMV promoter; a Kozak sequence is immediately upstream of the start codon.
PEPTIDE TAG
The N-terminal CL7 tag is between the His8 and eGFP tags, and a PreScission protease (PSC) cleavage site is C-terminal to all tags.
CLEAVAGE SITE(S)
C-terminal PSC P
OTHER TAGS
His8 tag is N-terminal, followed by CL7, then eGFP.
VOLUME | 10 ug at 500 ng/uL
EXPIRATION | 12 months from receipt when stored as directed
Plasmid Sequences are available on the Plasmid Guide Page
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