TriAltus' highly pure, tag-free Phi29 DNA polymerase is a highly processive, isothermal enzyme from Bacillus subtilis phage phi29, known for its exceptional strand displacement and ability to synthesize long DNA fragments up to 70 kb. With a robust 3'→5' proofreading exonuclease function, it ensures high-fidelity amplification, making it ideal for applications like whole genome amplification and DNA sequencing preparation.
SPECIFICATIONS
PURITY | ≥95%
ENZYME SOURCE | Phi29 DNA polymerase (from Bacillus subtilis phage phi29) is expressed in E. coli and is purified with our CLīM technology.
STORAGE/SHIPPING CONCENTRATION | 10 U/µL
STORAGE BUFFER | 10 mM Tris-Cl pH 7.4 (25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% Tergitol 15-S-40, 50% glycerol
RECOMMENDED STORAGE CONDITIONS | -20°C
EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.
SKU |
3,000 Units: 40-2414
10,000 Units: 40-2420
Customize concentration and scale to meet your specific research needs. Please Inquire.
One unit is defined as the amount of enzyme required to convert 0.5 pmol dNTPs to acid-insoluble material in 10 min at 30°C.
Phi29 DNA Polymerase Data Sheet
Use of TriAltus products falls under our limited license agreement and is for non-profit research use only. For commercial use, please contact us for licensing.