The One-Shot Trick for "Impossible" Multi-Subunit Proteins

The One-Shot Trick for "Impossible" Multi-Subunit Proteins

Multi-subunit giants like bacterial RNA polymerases haunt structural biologists and assay developers. Why? They're finicky—coordinated expression, tricky assembly, ultra-sensitive to conditions. Most labs burn 3–5 chromatography steps just to scrape by with usable material.

TriAltus' CL7/Im7 Game-Changer

We cracked it with our CL7/Im7 one-step system + custom vectors. Targeted T. thermophilus and M. tuberculosis RNA polymerases? Done.

Single T7 promoter drives all subunits in monocistronic order—synchronous translation, perfect in vivo assembly. CL7 tag on beta-prime N-terminus locks it to Im7 resin. One capture.

Results That Slash Your Workflow

• >99% Purity: SDS-PAGE + silver stain shows zero background. Noise-free kinetics, binding assays, crystallography.

• Perfect Stoichiometry: No co-transfection chaos or multi-vector imbalances. Full complexes, no truncations.

• Higher Yields: True one-step capture/release skips ion exchange/SEC. More active enzyme, faster.

• Protease-Safe: On-resin PreScission cleavage cuts time and exposure. Native activity preserved.

Who Wins Big

• Structural biologists scaling homogeneous complexes for cryo-EM/X-ray.

• Transcription/CRISPR researchers demanding intact multimers.

• Assay teams killing background interference in RNP workflows.

Skip the grind. Outsource to TriAltus CLīM™—your RNA pol arrives pure, assembled, ready.