Starter Kit: 1-mL Gravity Flow Column - Pre-Loaded Im7 Resin

Starter Kit: 1-mL Gravity Flow Column - Pre-Loaded Im7 Resin

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The 1-mL Gravity Flow Column - Pre-Loaded Im7 Resin Starter Kit provides all essential buffers and reagents necessary for the purification of CL7 tagged proteins. Please select a plasmid and an elution protease.


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The Im7 1-mL Gravity Flow Column Starter Kit consists of:

• Equilibration, Lysis, Elution Buffer (100 mL)
• Wash Buffer A1 (High Salt) (100 mL)
• Wash Buffer A2 (No Salt) (100 mL)
• 1-mL Gravity Flow Column - Pre-Loaded Im7 Resin (1 mL)
• Elution Protease (1 mg)
• Plasmid (100 ng; 30 ng/µL)
Short Protocol

Please refer to the comprehensive protocols for the exact purification protocol and Lysis and Elution buffer preparation.

SPECIFICATIONS (Im7 Resin)


PARTICLE SIZE | Crosslinked agarose 6B beads (45 – 165 μM)


PH STABILITY | The Im7 protein is stable on the beads at pH 3-10. However, CL7/Im7 binding is stable at pH 4.2 – 10 only.


SALT STABILITY |  4M NaCl tested


BINDING CAPACITY | 35-40 mg CL7/mL resin


STORAGE/SHIPPING CONCENTRATION | 50/50 buffer/resin slurry. Available in 1-mL and 5-mL columns.

 

RECOMMENDED OPERATING TEMPERATURE | 4°C or room temperature


REACTIVATION DETAILS | To remove the CL7 protein and reactivate the resin, wash the column with Gdn-HCl, exchanging it into physiological buffer.


Additional information: Target protein characteristics (e.g., protein size, conformation, and concentration); flow rate (i.e., lower flow rates may increase the binding capacity); and other parameters (e.g., pH and temperature) can affect the column binding capacity.