activated im7 resin
activated im7 resin
Activated Im7 Resin
Activated Im7 Resin
Activated Im7 Resin
Activated Im7 Resin
Activated Im7 Resin
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  • Load image into Gallery viewer, Activated Im7 Resin
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Activated Im7 Resin

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$140.00
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$140.00
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The Activated Im7 Resin contains a highly specific, resilient Im7 domain that binds the CL7 tag (~16 kDa) fused to target proteins. Following cleavage with the appropriate protease, the target protein releases from the Im7-bound CL7. Available in 1-, 2-, 5-, and 15-mL volumes of settled resin (50% suspension).

Capable of up to 100 reactivations, TriAltus’s Activated Im7 Resin retains a 1:1 molar binding ratio during repeated uses. The Activated Im7 Resin has been used to purify a variety of proteins – including membrane and multisubunit proteins.

SIMPLICITY – In a single purification step, this protocol yields purified proteins that exhibit high yield, high purity, and a high activity level.


REUSABILITY – The Im7 resin is reusable with no observable loss in capacity.


RESILIENCYThe Im7 Resin retains a 1:1 molar binding ratio after multiple reactivations in a variety of conditions, including various salt and detergent concentrations.


SPECIFICATIONS


BUFFER | 20 mM Tris-Cl pH 8.0, 0.5M NaCl, 5% glycerol, 0.05% sodium azide

 

PARTICLE SIZE | Crosslinked agarose 6B beads (45 – 165 μM)


PH STABILITY | The Im7 protein is stable on the beads at pH 3-10. However, CL7/Im7 binding is stable at pH 4.2 – 10 only.


SALT STABILITY | 4M NaCl tested


BINDING CAPACITY | 35-40 mg CL7/mL resin


STORAGE/SHIPPING CONCENTRATION | 50/50 buffer/resin slurry. 1-, 2-, 5-, and 15-mL of settled resin in 2, 4, 10, and 30 mL of slurry, respectively.

 

RECOMMENDED OPERATING TEMPERATURE | 4°C or room temperature


REACTIVATION DETAILS | To remove the CL7 protein and reactivate the resin, wash the column with Gdn-HCl, exchanging it into physiological buffer.


EXPIRATION | 12 months from receipt when stored as directed

Additional information: Target protein characteristics (e.g., protein size, conformation, and concentration); flow rate (i.e., lower flow rates may increase the binding capacity); and other parameters (e.g., pH and temperature) can affect the column binding capacity. Protocols and data sheets are available for download under the Support tab.

Use of TriAltus products falls under our limited license agreement and is for non-profit research use only. For commercial use, please contact us for licensing.