The Activated Im7 Resin contains a highly specific, resilient Im7 domain that binds the CL7 tag (~16 kDa) fused to target proteins. Following cleavage with the appropriate protease, the target protein releases from the Im7-bound CL7. Available in 1-, 2-, 5-, and 15-mL volumes of settled resin (50% suspension).
Capable of up to 100 reactivations, TriAltus’s Activated Im7 Resin retains a 1:1 molar binding ratio during repeated uses. The Activated Im7 Resin has been used to purify a variety of proteins – including membrane and multisubunit proteins.
SIMPLICITY – In a single purification step, this protocol yields purified proteins that exhibit high yield, high purity, and a high activity level.
REUSABILITY – The Im7 resin is reusable with no observable loss in capacity.
RESILIENCY – The Im7 Resin retains a 1:1 molar binding ratio after multiple reactivations in a variety of conditions, including various salt and detergent concentrations.
BUFFER | 20 mM Tris-Cl pH 8.0, 0.5M NaCl, 5% glycerol, 0.05% sodium azide
PARTICLE SIZE | Crosslinked agarose 6B beads (45 – 165 μM)
PH STABILITY | The Im7 protein is stable on the beads at pH 3-10. However, CL7/Im7 binding is stable at pH 4.2 – 10 only.
SALT STABILITY | ≤ 4M NaCl tested
BINDING CAPACITY | 35-40 mg CL7/mL resin
STORAGE/SHIPPING CONCENTRATION | 50/50 buffer/resin slurry. 1-, 2-, 5-, and 15-mL of settled resin in 2, 4, 10, and 30 mL of slurry, respectively.
RECOMMENDED OPERATING TEMPERATURE | 4°C or room temperature
REACTIVATION DETAILS | To remove the CL7 protein and reactivate the resin, wash the column with Gdn-HCl, exchanging it into physiological buffer.
Additional information: Target protein characteristics (e.g., protein size, conformation, and concentration); flow rate (i.e., lower flow rates may increase the binding capacity); and other parameters (e.g., pH and temperature) can affect the column binding capacity. Protocols and data sheets are available for download under the Support tab.