TriAltus’ highly pure Cas9 HiFi protein is used for genome editing with CRISPR technology. Recombinant HiFi Streptococcus pyogenes Cas9 protein is a high fidelity variant of Cas9 with reduced off-target effects compared to WT protein. It is expressed in E. coli with two (N-terminal and C-terminal) nuclear localization signals (NLS) and the CL7 tag, which is removed with PreScission protease during the purification process. Cas9 HiFi protein combines with guide RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex that enables efficient and precise genome editing in cells.
PURITY | ~99% (SDS-PAGE gel analysis)
ENZYME SOURCE | Streptococcus pyogenes Cas9 HiFi is a single point mutant expressed in E. coli with two (N-terminal and C-terminal) nuclear localization signals (NLS), and is purified with our CL7 tag technology.
STORAGE/SHIPPING CONCENTRATION | 5 mg/mL
STORAGE BUFFER |10 mM Tris-Cl pH 8.0, 250 mM NaCl, 50% glycerol
RECOMMENDED STORAGE CONDITIONS | -80°C