The PreScission protease (PSC) recognizes and cleaves the sequence LEVLFQ/GP (where “/” indicates the cleavage site) to release the target protein from the Im7-bound CL7 tag. We ship this protease on dry ice in buffer (20 mM Tris (pH 8.0), 0.5 M NaCl, 1 mM EDTA, 50% glycerol).
PURITY | >98% (SDS gel analysis)
ENZYME SOURCE | Human Rhinovirus (HRV) 3C Protease overexpressed in E. coli with a glutathione S-transferase (GST) tag
CLEAVAGE SITE | PSC recognizes the sequence LEVLFQGP and cleaves between the glutamine (Q) and glycine (G)
CLEAVAGE EFFICIENCY | ~99% at 0.1 mg protease/mg target protein
STORAGE/SHIPPING CONCENTRATION | ~5 mg/mL
RECOMMENDED ASSAY CONCENTRATION | 2 mg/mL
RECOMMENDED STORAGE CONDITIONS | -80°C long term or -20°C for 2-3 months in shipping buffer
Additional processing information: After a CL7-tagged protein binds the activated Im7 resin, PSC is applied to the column to release the protein from the CL7 tag. The final protease concentration in the eluted sample comprises ~1.5-3% of total impurities. If needed, PSC can be purified from the sample with a GST-trap column or size exclusion chromatography.
TriAltus offers several exclusive expression plasmids with the PSC cleavage site and CL7 tag, including Plasmid #9, 10, 21, 31, 32, 33, and 34.