TriAltus optimized this plasmid for the expression of membrane proteins with C-terminal His8 and CL7 tags. Plasmid #9 encodes YidC – inserted at BamHi/SpeI restriction sites – as a model CL7-tagged membrane protein.
Transcription is driven by the T7 RNA polymerase, and the plasmid is designed for expression in E. Coli.
The CL7 tag for purification is located at the C-terminus, downstream of a PreScission protease (PSC) cleavage site and His8 tag.
Plasmid #9 includes a PSC cleavage site directly adjacent to the YidC model protein and upstream of both the His8 and CL7 purification tags.
The plasmid contains a His8 tag, downstream of the model protein and cleavage tag and upstream of the CL7 tag.