Plasmid #34 enables target proteins to be labeled with C-terminal His8 and CL7.
Transcription is driven by the T7 RNA polymerase, and the plasmid is designed for expression in E. Coli.
The C-terminal CL7 tag is downstream of Sortase A (SRT) and PreScission (PSC) cleavage sites and a His8 tag.
N-terminal SUMO and C-terminal SRT and PSC cleavage sites are available.
The encoded region begins with a Trx tag and includes a C-terminal His8 tag upstream of CL7.