Plasmid #32 enables target proteins to be labeled with N-terminal His8 and C-terminal CL7.
Transcription is driven by the T7 RNA polymerase, and the plasmid is designed for expression in E. Coli.
The C-terminal CL7 tag is downstream of three PreScission (PSC) and one Sortase A (SRT) cleavage site.
An N-terminal SUMO cleavage site exists upstream of the His8 tag. One SRT and three PSC cleavage sites exist between the target protein and the CL7 tag.
This plasmid includes N-terminal Trx and His8 tags.