Plasmid #31 enables target proteins to be labeled with N-terminal His8 and C-terminal CL7.
Transcription is driven by the T7 RNA polymerase, and the plasmid is designed for expression in E. Coli.
The N-terminal CL7 tag is downstream of three PreScission (PSC) and one Sortase A (SRT) cleavage site.
Plasmid #31 include a C-terminal SUMO cleavage site, upstream of the His8 tag; a TEV site between the His8 tag and target protein; a SRT site downstream of the target protein; and three PSC cleavage sites between the SRT and CL7 tag.
This plasmid includes N-terminal Trx and His8 tags.