ACTIVATED IM7 RESIN
The TriAltus affinity purification resin contains a highly specific, resilient Im7 domain that binds the CL7 tag (~16 kDa) fused to target proteins. Following cleavage with the appropriate protease, the target protein is released from the Im7-bound CL7. Capable of up to 100 reactivations, TriAltus’s Im7 Resin retains a 1:1 molar binding ratio during repeated uses. The Im7 Resin has been used to purify a variety of proteins – including membrane and multisubunit proteins – and is available in 2-, 4-, or 10-mL volumes.
FEATURES OF TRIALTUS IM7 RESIN:
SIMPLICITY – In a single purification step, this protocol yields purified proteins that exhibit high yield, high purity, and a high activity level.
REUSABILITY – The resin can be reused with no observable loss in capacity.
RESILIENCY – The Im7 Resin retains a 1:1 molar binding ratio after multiple reactivations in a variety of conditions, including various salt and detergent concentrations.
PARTICLE SIZE | Crosslinked agarose 6B beads (45 – 165 μM)
PH STABILITY | The Im7 protein is stable on the beads at pH 3-10; however, CL7/Im7 binding is stable at pH 4.2 – 10 only.
SALT STABILITY | ≤ 4M NaCl tested
BINDING CAPACITY | 18-22 mg CL7/mL resin
RECOMMENDED OPERATING TEMPERATURE | 4°C or room temperature
REACTIVATION DETAILS | To remove the CL7 protein and reactivate the resin, wash the column with guanidine hydrochloride, exchanging it into physiological buffer.