Plasmid Guide Need help picking the right plasmid? Here's some advice on where to start.
All E. coli expression vectors have: 1. Kanamycin resistance 2. T7 promoter 3. Overexpression induced with IPTG
Plasmid Selection Charts Refer to the charts below for detailed breakdowns of each plasmid's components.
Your plasmid choice depends on which terminus you want to tag and whether you want to add a His tag or other expression tag (e.g. Trx, MBP, SUMO).
Test Substrate Plasmid
For expression of CL7-tagged model protein.
The CL7-tagged model protein CL7M is used as the test substrate for verifying the binding capacity of Im7 resin.