Plasmid Guide Need help picking the right plasmid? Here's some advice on where to start.

multisubunit protein

Multisubunit Proteins

Multisubunit proteins may work well with Plasmid 1.

 

 

large, multimeric protein

Large or Multimeric Proteins

Large or multimeric proteins may do well with one of the 30’s (Plasmid 31, 32, 33, or 34), since it has a long spacer arm with multiple protease sites to maximize chances of successful cleavage. 

Membrane Proteins

Membrane Proteins

For membrane proteins, Plasmid 9 & Plasmid 10 are good to try with the engineered signal peptide. 




All E. coli expression vectors have: 1. Kanamycin resistance      2. T7 promoter      3. Overexpression induced with IPTG

Plasmid Selection Charts Refer to the charts below for detailed breakdowns of each plasmid's components.

Your plasmid choice depends on which terminus you want to tag and whether you want to add a His tag or other expression tag (e.g. Trx, MBP, SUMO).

 
Group 1 Plasmids
For membrane proteins.
 
Group 20 Plasmids
N-terminal expression and CL7 tags.

These plasmids each have a TRX expression tag and a N-terminal CL7 tag cleavable by SUMO. The main difference between Plasmids 21 and 22 is that Plasmid 22 also has a C-terminal His-tag.

 
Group 30 Plasmids
N-terminal expression and C-terminal CL7 tags.

Group 30 Plasmids have a longer spacer arm between the target & the bead, so they are good for big or bulky proteins.

 
Multi-Subunit Plasmids
For expression of multiple subunits (ex. telomerase)
_edit6703A
 
Test Substrate Plasmid
For expression of CL7-tagged model protein. 

The CL7-tagged model protein CL7M is used as the test substrate for verifying the binding capacity of Im7 resin.

Group 1 Membrane Proteins

Group 20 N-Terminal Expression & CL7 Tags

Group 30 N-Terminal Expression & C-terminal CL7 Tags

Multi-Subunit Vectors

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