PURITY | >99% (SDS-PAGE gel analysis)
ENZYME SOURCE | TriAltus hGH is human recombinant protein produced from E. coli in soluble form purified using our CLiM technology.
EC50 (half-maximal effective concentration) | ~0.2 ng/mL measured by a cell proliferation assay using 32D-rGHR cells .
ENDOTOXIN | <0.1 EU/ug by rFC method (Arvys Proteins Inc.)
STORAGE/SHIPPING CONCENTRATION | 10-25 ug sizes are supplied at 200ug/mL in PBS. Larger sizes are lot-specific and bottled at the concentration indicated on the vial.
STORAGE BUFFER | PBS
SHIPPING CONDITIONS | Blue ice
RECOMMENDED STORAGE CONDITIONS | -80°C. Avoid repeated freeze-thaw cycles.
EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.
Figure 1. Human Growth Hormone induces proliferation of 32D-rGHR cells with an EC50 = ~0.2 ng/mL. (Insulin receptor substrate-1 enhances growth hormone-induced proliferation, Liang L, Zhou T, Jiang J, Pierce JH, Gustafson TA, Frank SJ.Endocrinology. 1999 May;140(5):1972-83. doi: 10.1210/endo.140.5.6724.PMID: 10218944).
Figure 2. hGH at >99% purity as purified with the CL7/Im7 system. R = reduced, NR = non-reduced.
1 mg: 40-2030
5 mg: 40-2035