TriAltus’ recombinant human growth hormone (hGH) is used for a broad range of non-therapeutic research purposes. hGH is produced from E. coli in soluble form and purified using our CL7 tag technology. hGH is usually mostly insoluble, but the CL7 tag increases its solubility and avoids the need to purify from inclusion bodies.
SPECIFICATIONS
PURITY | >99% (SDS-PAGE gel analysis)
ENZYME SOURCE | TriAltus hGH is human recombinant protein produced from E. coli in soluble form purified using our CL7 tag technology.
EC50 (half-maximal effective concentration) | ~0.2 ng/mL measured by a cell proliferation assay using 32D-rGHR cells .
ENDOTOXIN | <0.1 EU/ug by rFC method (Arvys Proteins Inc.)
STORAGE/SHIPPING CONCENTRATION | 10-25 ug sizes are supplied at 200ug/mL in PBS. Larger sizes are lot-specific and bottled at the concentration indicated on the vial.
STORAGE BUFFER | PBS
SHIPPING CONDITIONS | Blue ice
RECOMMENDED STORAGE CONDITIONS | -80°C. Avoid repeated freeze-thaw cycles.
EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.
Figure 1. Human Growth Hormone induces proliferation of 32D-rGHR cells with an EC50 = ~0.2 ng/mL. (Insulin receptor substrate-1 enhances growth hormone-induced proliferation, Liang L, Zhou T, Jiang J, Pierce JH, Gustafson TA, Frank SJ.Endocrinology. 1999 May;140(5):1972-83. doi: 10.1210/endo.140.5.6724.PMID: 10218944).
Figure 2. hGH at >99% purity as purified with the CL7/Im7 system. R = reduced, NR = non-reduced.
hGH Data Sheet